Next Generation Sequencing for museum scientists

This guide is not meant to be a comprehensive collection of all things NGS. Rather, this guide is meant to be an introduction to Next Generation Sequencing to museum scientists or other researchers who study similar questions in evolution, taxonomy, systematics, and biodiversity. This also focuses on “non-model” organisms which often do not have established genomic resources. This means that many methods more commonly used in an academic setting but not in a museum setting may not be included at this time. This guide is also a way for me to keep track of my own notes and resources I find.

How to navigate this guide

Perhaps you are looking through this guide because you want to start a new project, or maybe better understand the methods you are using, or just generally curious. This should cater to all of those, though you may navigate through the guide a little differently. I will start with considerations to design a project and below it there will be a visual pipeline of the molecular methods. I will also include an outline and glossary so one can simply find the page they are looking for. You can either follow along the pipeline or jump directly to the page of interest.

Designing a project

Before diving into the methods, one must first ask

Molecular lab work pipeline